goat polyclonal anti cd4  (R&D Systems)

Journal: Biology

Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema

doi: 10.3390/biology10090934

Figure Lengend Snippet: Inclusion and exclusion criteria.

Article Snippet: Primary antibodies for immunohistochemical staining included rabbit polyclonal anti-human collagen I (ab34170; Abcam, Cambridge, UK), rabbit polyclonal anti-human Ki67 (ab15580; Abcam), goat polyclonal anti-human CD4 (AF379; R&D Systems, Minneapolis, MN, USA), goat polyclonal anti-human LYVE-1 (AF2089; R&D Systems), rabbit polyclonal anti-human periostin (ab14041; Abcam), rabbit polyclonal anti-human IL13 (ab9576; Abcam), mouse monoclonal anti-human mast cell tryptase (ab2378; Abcam), rabbit polyclonal anti-human thymic stromal lymphopoietin (TSLP; ab188766; Abcam), guinea pig polyclonal anti-human cytokeratin14 (ab192694; Abcam), goat polyclonal anti-human IL33 (AF3625; R&D System), rat monoclonal anti-IL25 (NBP2-11677; Novus Biologicals, Centennial, Colorado), rabbit polyclonal anti-human IL13 receptor (ab79277; Abcam), mouse monoclonal anti-human IL4 (MAB304; R&D Systems), and mouse monoclonal anti-human IL5 (MAB605; R&D Systems).

Techniques: Dissection, Bioprocessing

Journal: Biology

Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema

doi: 10.3390/biology10090934

Figure Lengend Snippet: Treatment with QBX258 decreases mast cell infiltration in lymphedematous skin. ( a ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue) and CD4 + cells (pink) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. ( b ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue), LYVE-1 (red), and tryptase (green) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. Scale bar (low-power images): 200 μm; scale bar (magnified images): 25 μm; * p < 0.05; ** p < 0.01.

Article Snippet: Primary antibodies for immunohistochemical staining included rabbit polyclonal anti-human collagen I (ab34170; Abcam, Cambridge, UK), rabbit polyclonal anti-human Ki67 (ab15580; Abcam), goat polyclonal anti-human CD4 (AF379; R&D Systems, Minneapolis, MN, USA), goat polyclonal anti-human LYVE-1 (AF2089; R&D Systems), rabbit polyclonal anti-human periostin (ab14041; Abcam), rabbit polyclonal anti-human IL13 (ab9576; Abcam), mouse monoclonal anti-human mast cell tryptase (ab2378; Abcam), rabbit polyclonal anti-human thymic stromal lymphopoietin (TSLP; ab188766; Abcam), guinea pig polyclonal anti-human cytokeratin14 (ab192694; Abcam), goat polyclonal anti-human IL33 (AF3625; R&D System), rat monoclonal anti-IL25 (NBP2-11677; Novus Biologicals, Centennial, Colorado), rabbit polyclonal anti-human IL13 receptor (ab79277; Abcam), mouse monoclonal anti-human IL4 (MAB304; R&D Systems), and mouse monoclonal anti-human IL5 (MAB605; R&D Systems).

Techniques: Staining

Journal: Science immunology

Article Title: Clonal expansion and activation of tissue-resident memory-like Th17 cells expressing GM-CSF in the lungs of severe COVID-19 patients.

doi: 10.1126/sciimmunol.abf6692

Figure Lengend Snippet: Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded CD4+ T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.

Article Snippet: Immunofluorescence microscopy was performed in 1-m paraffin-embedded sections, after 15-min antigen retrieval with pH 9 antigen retrieval solution (Agilent, Santa Clara, CA, USA) and incubation with polyclonal primary goat anti-CD4 antibody (R&D Systems, Minneapolis, MN, USA, AF-379) and rabbit anti-CCR6 antibody (Abcam, Cambridge, UK, ab140768).

Techniques: Activation Assay, Gene Expression, Comparison, Clone Assay, Migration

Journal: Science immunology

Article Title: Clonal expansion and activation of tissue-resident memory-like Th17 cells expressing GM-CSF in the lungs of severe COVID-19 patients.

doi: 10.1126/sciimmunol.abf6692

Figure Lengend Snippet: Fig. 5. Cytokine secretion profile and cellular source of GM-CSF. (A) GM-CSF and IL-17A protein in serum of patients with COVD-19 (n = 8) and healthy controls (n = 7) from Hamburg and of patients with moderate (n = 8) or severe COVID-19 (n = 11) from Halle as indicated. Cell map of (B) CSF2 (GM-CSF) expressing and (C) IL17A express- ing cells (scale bars indicate normalized expression). Three different UMAPs with different cellular granularity showing the respective gene expression of in total cells of the BALF (left), total T cells of blood and BALF (middle), and total T cells in BALF (right) from all patients. (D) Immunofluorescence of CD4+ (green) CCR6+ (red) TRM17 cells in the lungs of a deceased patient with COVID-19 infection [nuclear staining 4′,6-diamidino-2-phenylindol (DAPI), blue] (two additional samples are presented in fig. S10B). (E) Combined immunofluorescence (CCR6) and FISH (IL17A) of lung samples from one patient with COVID-19. (F) Concentrations of the indicated cytokines in the BALF of patients with COVID-19 and bacterial pneumonia.

Article Snippet: Immunofluorescence microscopy was performed in 1-m paraffin-embedded sections, after 15-min antigen retrieval with pH 9 antigen retrieval solution (Agilent, Santa Clara, CA, USA) and incubation with polyclonal primary goat anti-CD4 antibody (R&D Systems, Minneapolis, MN, USA, AF-379) and rabbit anti-CCR6 antibody (Abcam, Cambridge, UK, ab140768).

Techniques: Expressing, Gene Expression, Immunofluorescence, Infection, Staining

Journal: Frontiers in Immunology

Article Title: The Reversal of Immune Exclusion Mediated by Tadalafil and an Anti-tumor Vaccine Also Induces PDL1 Upregulation in Recurrent Head and Neck Squamous Cell Carcinoma: Interim Analysis of a Phase I Clinical Trial

doi: 10.3389/fimmu.2019.01206

Figure Lengend Snippet: Tumors from patients treated with Tadalafil and MUC1/polyICLC vaccine show a lower infiltration of MDSCs and Treg and a higher infiltration of activated CD8 in the tumor bed. Computer based image cytometry was performed to enumerate the number of (A) MDSC, (B) IL4Rα − myeloid cells, (C) CD4 + T cell subsets, or (D) CD8 + T cells. (E) CD69 expression within the CD8 is reported normalized on the CD69 expression on all the cells evaluated. Depending on the region of interest evaluated, at least 10 5 -10 6 cells were analyzed. (F) The expression of CD69 in CD8 + T cells was plotted against MUC1 IHC score of the corresponding tumor. Two ways T -test and relevant pearson correlation parameters are reported.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-human FOXP3 antibody (clone 237/E7, dilution 1/25, Abcam) and the goat polyclonal anti-human CD4 antibody, (dilution 1/20, R&D System).

Techniques: Cytometry, Expressing